Gender issues are some of the core concerns of policy, practice and provision in relation to education. There is a popular notion that boys are naughty while girls are good mannered in primary schools. In many studies, boys were found to engage in more rough-and tumble play and physical fighting than do girls (Thorne, 2002). It seems that it is more difficult for teachers to deal with boys than with girls. However, in the emerging literature, within girls, there are different types of emotional and behavioral problems, such as aggression, which is not unusual that teachers may face with. Research with empirical data on how teachers help girls with emotional and behavioral problems is spare. In order to understand more how teachers in training deal with the gender and the nature of students' problem, this study investigates the responses of pre-service teachers in helping students in a guidance training programme, which is a part of a pre-service new B.Ed. module. The study aims to make some suggestions for effective preparation of the pre-service teachers to play their guidance role in Hong Kong primary schools. In an attempt to explore how pre-service teachers developed their capacity in helping students with different gender and nature of problems, two scenarios were used. Case 1 was a by in a helpless situation (with emotional difficulties) while case 2 was an angry girl with aggressive behavior (with emotional and behavioural difficulties). Qualitative research approach in form of case study was used. The sample, 16 student teachers with some seemed to be very aware of, others largely ignorant about the guidance role of teacher were selected for in-depth interviews to chart their development in the training programme. Their responses for handling of two different cases of pupils' problems in the interview schedule were analyzed. The results of the study show that student teachers had developed their abilities in handling pupils' problems in terms of attitude towards