With the increasing concern in school teachers’ well-being and growing focus on the early childhood education sector in recent years, the present study explored the factors that contribute to the psychological well-being of kindergarten teachers in Hong Kong. Based on a sample of 459 kindergarten teachers in Hong Kong, the authors demonstrated that teachers’ psychological well-being was strongly determined by their self-belief, which were contributed by their relationship with colleagues, and their positivity in attitude towards life and work. The authors further demonstrated that teachers’ self-belief was influenced by their perception of the school culture. Implications for establishing a positive attitude in kindergarten teachers and enhancing their psychological well-being were discussed. Copyright © 2021 The International Society for Quality-of-Life Studies (ISQOLS) and Springer Nature B.V.