Objectives: It has been supported by various literatures that there is a relation between holistic processing and Chinese character recognition. The present study examined the relation between holistic processing of Chinese characters and literacy ability of Hong Kong preschoolers, in order to explore whether the extent of holistic processing is associated with writing or reading experience. Methods: Participants were 59 local preschoolers (37 boys, 22 girls). The literacy ability of participants was assessed by The Hong Kong Reading Ability Screening Test for Preschool Children (RAST-K); while holistic processing of Chinese characters was measured by adopting the procedures from Hsiao & Cottrell (2009) in a complete composite paradigm (Gauthier & Bukach, 2007). Results: In line with the previous research findings, preschoolers also showed holistic processing in perceiving Chinese characters. Specifically, K2 students were significantly more holistic than K3 students. Such discrepancy may relate to writing experience instead of reading experience since a negative correlation between holistic processing and writing ability was significantly observed. Conclusion: This study had a theoretical contribution on the study of Chinese writing difficulties among preschoolers. Educators and parents may help enhancing the writing ability of preschoolers by leading them to perceive the characters separately, i.e. weakening the holistic processing of Chinese characters. All rights reserved.