In this study, we assessed the general marine environmental knowledge and attitudes of university students from eight public universities in Hong Kong. The Ocean Literacy Framework and revised New Ecological Paradigm (NEP) were used as tools for questionnaire development. Data were collected via in-person and online surveys. An in-person survey was conducted at the university canteen from 16 May to 24 May 2017, and an online survey was conducted via email from 1 May to 31 May 2017. A structured questionnaire was provided to interested students from different levels of study and majors. Data obtained from these surveys were summarized based on participants’ correct answers in the general knowledge section and five-point Likert scaling for attitude statements. Results show that Hong Kong university students possess moderate marine environmental knowledge and pro-environmental attitudes. Knowledge scores significantly correlate with demographic variables, such as major of study, gender, institution, and parents’ education. Students’ pro-environmental attitudes are associated with different factors, including participation in various marine recreational activities, taking marine-related courses, and attachment to conservative marine initiatives. The study results have implications regarding the advancement of marine environmental knowledge and the pro-environmental attitudes of university students, such as mapping a well-structured pathway for disseminating marine environmental knowledge, curricular involvement, and the development of an integrated web resource. Copyright © 2023 by the authors.