Background: Hong Kong students with physical disabilities were being seen as physically inactive compared with typically developed people or even people with other disabilities, such as visual impairment or intellectual disabilities. Lack of regular physical activity would lead to poor physical fitness, and yet is associated with a higher risk of being obese or other physical as well as mental health problems. Currently, a newly developed sport, sitting light volleyball, modified from the Paralympic sitting volleyball, is considered as suitable for disabled people with lower physical fitness, in which was able to increase fitness level and increase their physical activity.Objectives: The current study aimed to examine the effect of sitting light volleyball in improving physical fitness among students with physical disabilities in Hong Kong.Methods: The current study was an interventional, quasi-experimental study. Nineteen participants, 8 males and 11 females, with the age of 18.5 on average (SD = 3.24), participated in the study. Two local special education schools were involved in the 10-week (65 minutes per session) sitting light volleyball intervention program. Participants took part in a fitness test before and after the intervention. The intended-to-treat approach was adopted throughout the data analysis, with the last observation carried forward method for managing the missing data.Results: No significant changes in body composition outcomes of the participants were found. The non-parametric sign test showed significant median differences on dumbbell press test and flexibility test of the right shoulder before and after the intervention, with Z = 2.94, P = 0.003; Z = 2.20, P = 0.028, respectively.Conclusions: This indicated that the sitting light volleyball intervention was able to provide preliminary evidence on showing the enhancement of muscle endurance and flexibility of the students with physical disabilities. Whereas, intensified interventions should be